Phosphatidylinositol 3-kinase affects mitochondrial function in part through inducing peroxisome proliferator-activated receptor γ coactivator-1β expression.

ABSTRACT:

Background and purpose

Hyperactivation of phosphatidylinositol 3-kinase (PI3K), commonly observed in cancer, is believed to promote cancer cell growth and survival. Appropriate mitochondrial function is an integral part of cellular function. How PI3K affects mitochondrial homeostasis is not fully understood.

Experimental approach

Mitochondrial mass, membrane potential and reactive oxygen species (ROS) were quantified by three different fluorogenic probes. Gene expression at the levels of mRNA and protein were measured by quantitative RT-PCR and Western analysis.

Key results

Using the PI3K inhibitors LY294002 and PI103, we found that suppressing PI3K activity altered mitochondrial function. Specifically, LY294002 and PI103 suppressed the mRNA expression levels of mitoc

SUBMITTER: Gao M 

PROVIDER: S-EPMC3042208 | biostudies-literature | 2011 Feb

REPOSITORIES: biostudies-literature