Segmental duplication as one of the driving forces underlying the diversity of the human immunoglobulin heavy chain variable gene region.

ABSTRACT:

Background

Segmental duplication and deletion were implicated for a region containing the human immunoglobulin heavy chain variable (IGHV) gene segments, 1.9III/hv3005 (possible allelic variants of IGHV3-30) and hv3019b9 (a possible allelic variant of IGHV3-33). However, very little is known about the ranges of the duplication and the polymorphic region. This is mainly because of the difficulty associated with distinguishing between allelic and paralogous sequences in the IGHV region containing extensive repetitive sequences. Inability to separate the two parental haploid genomes in the subjects is another serious barrier. To address these issues, unique DNA sequence tags evenly distributed within and flanking the duplicated region implicated by the previous studies were selected.

SUBMITTER: Pramanik S 

PROVIDER: S-EPMC3042411 | biostudies-literature | 2011 Jan

REPOSITORIES: biostudies-literature