Selectin-mediated recruitment of bone marrow stromal cells in the postischemic cerebral microvasculature.

ABSTRACT:

Background and purpose

The therapeutic potential of bone marrow stromal cells (BMSCs) has been demonstrated in different models of stroke. Although it is well established that BMSCs selectively migrate to the site of brain injury, the mechanisms underlying this process are poorly understood. This study addresses the hypothesis that selectins mediate the recruitment of BMSCs into the postischemic cerebral microvasculature.

Methods

Focal ischemic stroke was induced by middle cerebral artery occlusion and reperfusion. Cell recruitment was monitored using either fluorescent- or radiolabeled BMSCs detected by intravital microscopy or tissue radioactivity. Mice were treated with either a blocking antibody against P- or E-selectin or with the nonselective selectin antagonist, fucoi

SUBMITTER: Yilmaz G 

PROVIDER: S-EPMC3042505 | biostudies-literature | 2011 Mar

REPOSITORIES: biostudies-literature