Simultaneous measurement of protein oxidation and S-nitrosylation during preconditioning and ischemia/reperfusion injury with resin-assisted capture.

ABSTRACT:

Rationale

Redox modifications play an important role in many cellular processes, including cell death. Ischemic preconditioning (IPC) has been shown to involve redox signaling. Protein S-nitrosylation (SNO) is increased following myocardial IPC, and SNO is thought to provide cardioprotection, in part, by reducing cysteine oxidation during ischemia/reperfusion (IR) injury.

Objective

To test the hypothesis that SNO provides cardioprotection, in part, by shielding against cysteine oxidation following IR injury.

Methods and results

We developed a new method to measure protein oxidation using resin-assisted capture (Ox-RAC), which is similar to the SNO-RAC method used in the quantification of SNO. Langendorff-perfused hearts were subjected to various perfusion protocols (c

SUBMITTER: Kohr MJ 

PROVIDER: S-EPMC3042536 | biostudies-literature | 2011 Feb

REPOSITORIES: biostudies-literature