Allosteric response is both conserved and variable across three CheY orthologs.

ABSTRACT: A computational method to identify residues likely to initiate allosteric signals has been developed. The method is based on differences within stability and flexibility profiles between wild-type and perturbed structures as computed by a distance constraint model. Application of the approach to three bacterial chemotaxis protein Y (CheY) orthologs provides a comparison of allosteric response across protein family divergence. Interestingly, we observe a rich mixture of both conservation and variability within the identified allosteric sites. While similarity within the overall response parallels the evolutionary relationships, >50% of the best scoring putative sites are only identified in a single ortholog. These results suggest that detailed descriptions of intraprotein communication are

SUBMITTER: Mottonen JM 

PROVIDER: S-EPMC3042579 | biostudies-literature | 2010 Oct

REPOSITORIES: biostudies-literature