A dynamic notch injury response activates epicardium and contributes to fibrosis repair.

ABSTRACT:

Rationale

Transgenic Notch reporter mice express enhanced green fluorescent protein in cells with C-promoter binding factor-1 response element transcriptional activity (CBF1-RE(x)₄-EGFP), providing a unique and powerful tool for identifying and isolating "Notch-activated" progenitors.

Objective

We asked whether, as in other tissues of this mouse, EGFP localized and functionally tagged adult cardiac tissue progenitors, and, if so, whether this cell-based signal could serve as a quantitative and qualitative biosensor of the injury repair response of the heart.

Methods and results

In addition to scattered endothelial and interstitial cells, Notch-activated (EGFP(+)) cells unexpectedly richly populated the adult epicardium. We used fluorescence-activated cell sorting to i

SUBMITTER: Russell JL 

PROVIDER: S-EPMC3042596 | biostudies-literature | 2011 Jan

REPOSITORIES: biostudies-literature