Probiotic Lactobacillus rhamnosus downregulates FCER1 and HRH4 expression in human mast cells.

ABSTRACT:

Aim

To investigate the effects of four probiotic bacteria and their combination on human mast cell gene expression using microarray analysis.

Methods

Human peripheral-blood-derived mast cells were stimulated with Lactobacillus rhamnosus (L. rhamnosus) GG (LGG(®)), L. rhamnosus Lc705 (Lc705), Propionibacterium freudenreichii ssp. shermanii JS (PJS) and Bifidobacterium animalis ssp. lactis Bb12 (Bb12) and their combination for 3 or 24 h, and were subjected to global microarray analysis using an Affymetrix GeneChip(®) Human Genome U133 Plus 2.0 Array. The gene expression differences between unstimulated and bacteria-stimulated samples were further analyzed with GOrilla Gene Enrichment Analysis and Visualization Tool and MeV Multiexperiment Viewer-tool.

Results

LGG and Lc

SUBMITTER: Oksaharju A 

PROVIDER: S-EPMC3042653 | biostudies-literature | 2011 Feb

REPOSITORIES: biostudies-literature