De novo characterization of the gametophyte transcriptome in bracken fern, Pteridium aquilinum.

ABSTRACT:

Background

Because of their phylogenetic position and unique characteristics of their biology and life cycle, ferns represent an important lineage for studying the evolution of land plants. Large and complex genomes in ferns combined with the absence of economically important species have been a barrier to the development of genomic resources. However, high throughput sequencing technologies are now being widely applied to non-model species. We leveraged the Roche 454 GS-FLX Titanium pyrosequencing platform in sequencing the gametophyte transcriptome of bracken fern (Pteridium aquilinum) to develop genomic resources for evolutionary studies.

Results

681,722 quality and adapter trimmed reads totaling 254 Mbp were assembled de novo into 56,256 unique sequences (i.e. unigenes)

SUBMITTER: Der JP 

PROVIDER: S-EPMC3042945 | biostudies-literature | 2011 Feb

REPOSITORIES: biostudies-literature