Microgenomic analysis in skeletal muscle: expression signatures of individual fast and slow myofibers.

ABSTRACT:

Background

Skeletal muscle is a complex, versatile tissue composed of a variety of functionally diverse fiber types. Although the biochemical, structural and functional properties of myofibers have been the subject of intense investigation for the last decades, understanding molecular processes regulating fiber type diversity is still complicated by the heterogeneity of cell types present in the whole muscle organ.

Methodology/principal findings

We have produced a first catalogue of genes expressed in mouse slow-oxidative (type 1) and fast-glycolytic (type 2B) fibers through transcriptome analysis at the single fiber level (microgenomics). Individual fibers were obtained from murine soleus and EDL muscles and initially classified by myosin heavy chain isoform content. Gene e

SUBMITTER: Chemello F 

PROVIDER: S-EPMC3043066 | biostudies-literature | 2011 Feb

REPOSITORIES: biostudies-literature