Stoichiometry of HLA class II-invariant chain oligomers.

ABSTRACT:

Background

The HLA gene complex encodes three class II isotypes, DR, DQ, and DP. HLA class II molecules are peptide receptors that present antigens for recognition by T lymphocytes. In antigen presenting cells, the assembly of matched α and β subunits to heterodimers is chaperoned by invariant chain (Ii). Ii forms a homotrimer with three binding sites for class II heterodimers. The current model of class II and Ii structure states that three αβ heterodimers bind to an Ii trimer.

Methodology/principal findings

[corrected] We have now analyzed the composition and size of the complexes of class II and Ii using epitope tagged class II subunits and density gradient experiments. We show here that class II-Ii oligomers consist of one class II heterodimer associated with one Ii trim

SUBMITTER: Koch N 

PROVIDER: S-EPMC3043101 | biostudies-literature | 2011 Feb

REPOSITORIES: biostudies-literature