Enterohepatic helicobacter in ulcerative colitis: potential pathogenic entities?

ABSTRACT:

Background

Changes in bacterial populations termed "dysbiosis" are thought central to ulcerative colitis (UC) pathogenesis. In particular, the possibility that novel Helicobacter organisms play a role in human UC has been debated but not comprehensively investigated. The aim of this study was to develop a molecular approach to investigate the presence of Helicobacter organisms in adults with and without UC.

Methodology/principal findings

A dual molecular approach to detect Helicobacter was developed. Oligonucleotide probes against the genus Helicobacter were designed and optimised alongside a validation of published H. pylori probes. A comprehensive evaluation of Helicobacter genus and H. pylori PCR primers was also undertaken. The combined approach was then assessed in a ra

SUBMITTER: Thomson JM 

PROVIDER: S-EPMC3044171 | biostudies-literature | 2011 Feb

REPOSITORIES: biostudies-literature