An efficient algorithmic approach for mass spectrometry-based disulfide connectivity determination using multi-ion analysis.

ABSTRACT:

Background

Determining the disulfide (S-S) bond pattern in a protein is often crucial for understanding its structure and function. In recent research, mass spectrometry (MS) based analysis has been applied to this problem following protein digestion under both partial reduction and non-reduction conditions. However, this paradigm still awaits solutions to certain algorithmic problems fundamental amongst which is the efficient matching of an exponentially growing set of putative S-S bonded structural alternatives to the large amounts of experimental spectrometric data. Current methods circumvent this challenge primarily through simplifications, such as by assuming only the occurrence of certain ion-types (b-ions and y-ions) that predominate in the more popular dissociation methods,

SUBMITTER: Murad W 

PROVIDER: S-EPMC3044266 | biostudies-literature | 2011 Feb

REPOSITORIES: biostudies-literature