Overexpression of BCL2 enhances survival of human embryonic stem cells during stress and obviates the requirement for serum factors.

ABSTRACT: The promise of pluripotent stem cells as a research and therapeutic tool is partly undermined by the technical challenges of generating and maintaining these cells in culture. Human embryonic stem cells (hESCs) are exquisitely sensitive to culture conditions, and require constant signaling by growth factors and cell-cell and cell-matrix interactions to prevent apoptosis, senescence, and differentiation. Previous work from our laboratory demonstrated that overexpression of the prosurvival gene BCL2 in mouse embryonic stem cells overrode the requirement of serum factors and feeder cells to maintain mESCs in culture. To determine whether this prosurvival gene could similarly protect hESCs, we generated hESC lines that constitutively or inducibly express BCL2. We find that BCL2 overexpression

SUBMITTER: Ardehali R 

PROVIDER: S-EPMC3044421 | biostudies-literature | 2011 Feb

REPOSITORIES: biostudies-literature