Isolation and purification of Cu-free methanobactin from Methylosinus trichosporium OB3b.

ABSTRACT:

Background

The isolation of highly pure copper-free methanobactin is a prerequisite for the investigation of the biogeochemical functions of this chalkophore molecule produced by methane oxidizing bacteria. Here, we report a purification method for methanobactin from Methylosinus trichosporium OB3b cultures based on reversed-phase HPLC fractionation used in combination with a previously reported resin extraction. HPLC eluent fractions of the resin extracted product were collected and characterized with UV-vis, FT-IR, and C-1s NEXAFS spectroscopy, as well as with elemental analysis and ESI-MS.

Results

The results showed that numerous compounds other than methanobactin were present in the isolate obtained with resin extraction. Molar C/N ratios, mass spectrometry measurements,

SUBMITTER: Pesch ML 

PROVIDER: S-EPMC3044663 | biostudies-literature | 2011 Feb

REPOSITORIES: biostudies-literature