Responsiveness of genes to manipulation of transcription factors in ES cells is associated with histone modifications and tissue specificity.

ABSTRACT:

Background

In addition to determining static states of gene expression (high vs. low), it is important to characterize their dynamic status. For example, genes with H3K27me3 chromatin marks are not only suppressed but also poised for activation. However, the responsiveness of genes to perturbations has never been studied systematically. To distinguish gene responses to specific factors from responsiveness in general, it is necessary to analyze gene expression profiles of cells responding to a large variety of disturbances, and such databases did not exist before.

Results

We estimated the responsiveness of all genes in mouse ES cells using our recently published database on expression change after controlled induction of 53 transcription factors (TFs) and other genes. Respons

SUBMITTER: Sharov AA 

PROVIDER: S-EPMC3044670 | biostudies-literature | 2011 Feb

REPOSITORIES: biostudies-literature