Expanding the versatility of phage display I: efficient display of peptide-tags on protein VII of the filamentous phage.

ABSTRACT:

Background

Phage display is a platform for selection of specific binding molecules and this is a clear-cut motivation for increasing its performance. Polypeptides are normally displayed as fusions to the major coat protein VIII (pVIII), or the minor coat protein III (pIII). Display on other coat proteins such as pVII allows for display of heterologous peptide sequences on the virions in addition to those displayed on pIII and pVIII. In addition, pVII display is an alternative to pIII or pVIII display.

Methodology/principal findings

Here we demonstrate how standard pIII or pVIII display phagemids are complemented with a helper phage which supports production of virions that are tagged with octa FLAG, HIS(6) or AviTag on pVII. The periplasmic signal sequence required for pIII

SUBMITTER: Loset GA 

PROVIDER: S-EPMC3044727 | biostudies-literature | 2011 Feb

REPOSITORIES: biostudies-literature