Identification of reference genes across physiological states for qRT-PCR through microarray meta-analysis.

ABSTRACT:

Background

The accuracy of quantitative real-time PCR (qRT-PCR) is highly dependent on reliable reference gene(s). Some housekeeping genes which are commonly used for normalization are widely recognized as inappropriate in many experimental conditions. This study aimed to identify reference genes for clinical studies through microarray meta-analysis of human clinical samples.

Methodology/principal findings

After uniform data preprocessing and data quality control, 4,804 Affymetrix HU-133A arrays performed by clinical samples were classified into four physiological states with 13 organ/tissue types. We identified a list of reference genes for each organ/tissue types which exhibited stable expression across physiological states. Furthermore, 102 genes identified as reference g

SUBMITTER: Cheng WC 

PROVIDER: S-EPMC3044736 | biostudies-literature | 2011 Feb

REPOSITORIES: biostudies-literature