Expanding the versatility of phage display II: improved affinity selection of folded domains on protein VII and IX of the filamentous phage.

ABSTRACT:

Background

Phage display is a leading technology for selection of binders with affinity for specific target molecules. Polypeptides are normally displayed as fusions to the major coat protein VIII (pVIII) or the minor coat protein III (pIII). Whereas pVIII display suffers from drawbacks such as heterogeneity in display levels and polypeptide fusion size limitations, toxicity and infection interference effects have been described for pIII display. Thus, display on other coat proteins such as pVII or pIX might be more attractive. Neither pVII nor pIX display have gained widespread use or been characterized in detail like pIII and pVIII display.

Methodology/principal findings

Here we present a side-by-side comparison of display on pIII with display on pVII and pIX. Polypeptides

SUBMITTER: Loset GA 

PROVIDER: S-EPMC3044770 | biostudies-literature | 2011 Feb

REPOSITORIES: biostudies-literature