Transcriptome dynamics and molecular cross-talk between bovine oocyte and its companion cumulus cells.

ABSTRACT:

Background

The bi-directional communication between the oocyte and its companion cumulus cells (CCs) is crucial for development and functions of both cell types. Transcripts that are exclusively expressed either in oocytes or CCs and molecular mechanisms affected due to removal of the communication axis between the two cell types is not investigated at a larger scale. The main objectives of this study were: 1. To identify transcripts exclusively expressed either in oocyte or CCs and 2. To identify those which are differentially expressed when the oocyte is cultured with or without its companion CCs and vice versa.

Results

We analyzed transcriptome profile of different oocyte and CC samples using Affymetrix GeneChip Bovine Genome array containing 23000 transcripts. Out of 131

SUBMITTER: Regassa A 

PROVIDER: S-EPMC3045333 | biostudies-literature | 2011 Jan

REPOSITORIES: biostudies-literature