Identification of epigenetically regulated genes that predict patient outcome in neuroblastoma.

ABSTRACT:

Background

Epigenetic mechanisms such as DNA methylation and histone modifications are important regulators of gene expression and are frequently involved in silencing tumor suppressor genes.

Methods

In order to identify genes that are epigenetically regulated in neuroblastoma tumors, we treated four neuroblastoma cell lines with the demethylating agent 5-Aza-2'-deoxycytidine (5-Aza-dC) either separately or in conjunction with the histone deacetylase inhibitor trichostatin A (TSA). Expression was analyzed using whole-genome expression arrays to identify genes activated by the treatment. These data were then combined with data from genome-wide DNA methylation arrays to identify candidate genes silenced in neuroblastoma due to DNA methylation.

Results

We present eight g

SUBMITTER: Caren H 

PROVIDER: S-EPMC3045360 | biostudies-literature | 2011 Feb

REPOSITORIES: biostudies-literature