A de novo expression profiling of Anopheles funestus, malaria vector in Africa, using 454 pyrosequencing.

ABSTRACT:

Background

Anopheles funestus is one of the major malaria vectors in Africa and yet there are few genomic tools available for this species compared to An. gambiae. To start to close this knowledge gap, we sequenced the An. funestus transcriptome using cDNA libraries developed from a pyrethroid resistant laboratory strain and a pyrethroid susceptible field strain from Mali.

Results

Using a pool of life stages (pupae, larvae, adults: females and males) for each strain, 454 sequencing generated 375,619 reads (average length of 182 bp). De novo assembly generated 18,103 contigs with average length of 253 bp. The average depth of coverage of these contigs was 8.3. In total 20.8% of all reads were novel when compared to reference databases. The sequencing of the field strain gener

SUBMITTER: Gregory R 

PROVIDER: S-EPMC3045460 | biostudies-literature | 2011 Feb

REPOSITORIES: biostudies-literature