The cumulus cell gene expression profile of oocytes with different nuclear maturity and potential for blastocyst formation.

ABSTRACT:

Purpose

Gene expression in human ART cumulus cell (CC) has been related to oocyte maturity and competence but requires further validation. Expression dynamics were investigated in CC of oocytes at different maturational stages and with different developmental competence in a standard in vivo mouse superovulation model.

Methods

Quantitative PCR analysis of Has2, Vcan, Sdc4, Alcam, Grem1, Ptgs1 and Ptgs2 in CC collected at regular time intervals from 0 to 24 h post hCG injection.

Results

Three expression patterns were observed each with strong regulation (4-230× differences). Immediately prior to ovulation CC of GVBD oocytes have 5× less Sdc4 and Ptgs1 and 5× more Ptgs2 when compared to the CC of freshly ovulated PB oocytes. When compared to the latter, the post-ovulato

SUBMITTER: Adriaenssens T 

PROVIDER: S-EPMC3045483 | biostudies-literature | 2011 Jan

REPOSITORIES: biostudies-literature