Alternative splicing enriched cDNA libraries identify breast cancer-associated transcripts.

ABSTRACT:

Background

Alternative splicing (AS) is a central mechanism in the generation of genomic complexity and is a major contributor to transcriptome and proteome diversity. Alterations of the splicing process can lead to deregulation of crucial cellular processes and have been associated with a large spectrum of human diseases. Cancer-associated transcripts are potential molecular markers and may contribute to the development of more accurate diagnostic and prognostic methods and also serve as therapeutic targets. Alternative splicing-enriched cDNA libraries have been used to explore the variability generated by alternative splicing. In this study, by combining the use of trapping heteroduplexes and RNA amplification, we developed a powerful approach that enables transcriptome-wide expl

SUBMITTER: Ferreira EN 

PROVIDER: S-EPMC3045797 | biostudies-literature | 2010 Dec

REPOSITORIES: biostudies-literature