Identification of errors introduced during high throughput sequencing of the T cell receptor repertoire.

ABSTRACT:

Background

Recent advances in massively parallel sequencing have increased the depth at which T cell receptor (TCR) repertoires can be probed by >3log10, allowing for saturation sequencing of immune repertoires. The resolution of this sequencing is dependent on its accuracy, and direct assessments of the errors formed during high throughput repertoire analyses are limited.

Results

We analyzed 3 monoclonal TCR from TCR transgenic, Rag-/- mice using Illumina® sequencing. A total of 27 sequencing reactions were performed for each TCR using a trifurcating design in which samples were divided into 3 at significant processing junctures. More than 20 million complementarity determining region (CDR) 3 sequences were analyzed. Filtering for lower quality sequences diminished but did

SUBMITTER: Nguyen P 

PROVIDER: S-EPMC3045962 | biostudies-literature | 2011 Feb

REPOSITORIES: biostudies-literature