Differential gene expression and adherence of Escherichia coli O157:H7 in vitro and in ligated pig intestines.

ABSTRACT:

Background

Escherichia coli O157:H7 strain 86-24 grown in MacConkey broth (MB) shows almost no adherence to cultured epithelial cells but adheres well in pig ligated intestines. This study investigated the mechanisms associated with the difference between in-vitro and in-vivo adherence of the MB culture.

Methodology/principal findings

It was found that decreased adherence in vitro by bacteria grown in MB was mainly due to lactose, possibly implicating the involvement of carbon catabolite repression (CCR). Expression of selected virulence-related genes associated with adherence and CCR was then examined by quantitative PCR. When bacteria were grown in MB and Brain Heart Infusion with NaHCO(3) (BHIN) plus lactose, pH was reduced to 5.5-5.9 and there was a significant decrease

SUBMITTER: Yin X 

PROVIDER: S-EPMC3046156 | biostudies-literature | 2011 Feb

REPOSITORIES: biostudies-literature