Identification of anchor genes during kidney development defines ontological relationships, molecular subcompartments and regulatory pathways.

ABSTRACT: The development of the mammalian kidney is well conserved from mouse to man. Despite considerable temporal and spatial data on gene expression in mammalian kidney development, primarily in rodent species, there is a paucity of genes whose expression is absolutely specific to a given anatomical compartment and/or developmental stage, defined here as 'anchor' genes. We previously generated an atlas of gene expression in the developing mouse kidney using microarray analysis of anatomical compartments collected via laser capture microdissection. Here, this data is further analysed to identify anchor genes via stringent bioinformatic filtering followed by high resolution section in situ hybridisation performed on 200 transcripts selected as specific to one of 11 anatomical compartments within t

SUBMITTER: Thiagarajan RD 

PROVIDER: S-EPMC3046260 | biostudies-literature | 2011 Feb

REPOSITORIES: biostudies-literature