Caenorhabditis elegans chromosome arms are anchored to the nuclear membrane via discontinuous association with LEM-2.

ABSTRACT:

Background

Although Caenorhabditis elegans was the first multicellular organism with a completely sequenced genome, how this genome is arranged within the nucleus is not known.

Results

We determined the genomic regions associated with the nuclear transmembrane protein LEM-2 in mixed-stage C. elegans embryos via chromatin immunoprecipitation. Large regions of several megabases on the arms of each autosome were associated with LEM-2. The center of each autosome was mostly free of such interactions, suggesting that they are largely looped out from the nuclear membrane. Only the left end of the X chromosome was associated with the nuclear membrane. At a finer scale, the large membrane-associated domains consisted of smaller subdomains of LEM-2 associations. These subdomains were

SUBMITTER: Ikegami K 

PROVIDER: S-EPMC3046480 | biostudies-literature | 2010

REPOSITORIES: biostudies-literature