Data-directed top-down Fourier-transform mass spectrometry of a large integral membrane protein complex, Photosystem II from Galdieria sulphuraria

ABSTRACT: High-resolution top-down mass spectrometry was used to characterize eleven integral and five peripheral subunits of the 750 kDa Photosystem II (PSII) complex from the eukaryotic red alga, Galdieria sulphuraria. The primary separation used liquid chromatography mass spectrometry with concomitant fraction collection (LC-MS+) yielding around 40 intact mass tags (IMTs) at 100 ppm mass accuracy on a low-resolution electrospray-ionization mass spectrometer, whose retention and mass were used to guide subsequent high-resolution top-down nano-electrospray Fourier-transform ion-cyclotron resonance mass spectrometry experiments (FT-MS). Both collisionally activated and electron capture dissociation (CAD, ECD) were used to confirm the presence of eleven small subunits to mass accuracy within 5 ppm; P

SUBMITTER: Thangaraj B 

PROVIDER: S-EPMC3046778 | biostudies-literature | 2000 Jan

REPOSITORIES: biostudies-literature