Polymerase I and transcript release factor regulates lipolysis via a phosphorylation-dependent mechanism.

ABSTRACT:

Objective

Polymerase I and transcript release factor (PTRF) is a protein highly expressed in adipose tissue and is an integral structural component of caveolae. Here, we report on a novel role of PTRF in lipid mobilization.

Research design and methods

PTRF expression was examined in different adipose depots of mice during fasting, refeeding, and after administration of catecholamines and insulin. Involvement of PTRF during lipolysis was studied upon PTRF knockdown and overexpression and mutation of PTRF phosphorylation sites in 3T3-L1 adipocytes.

Results

PTRF expression in mouse white adipose tissue (WAT) is regulated by nutritional status, increasing during fasting and decreasing to baseline after refeeding. Expression of PTRF also is hormonally regulated because tre

SUBMITTER: Aboulaich N 

PROVIDER: S-EPMC3046836 | biostudies-literature | 2011 Mar

REPOSITORIES: biostudies-literature