Rapid analysis of seed size in Arabidopsis for mutant and QTL discovery.

ABSTRACT:

Background

Arabidopsis thaliana is a useful model organism for deciphering the genetic determinants of seed size; however the small size of its seeds makes measurements difficult. Bulk seed weights are often used as an indicator of average seed size, but details of individual seed is obscured. Analysis of seed images is possible but issues arise from variations in seed pigmentation and shadowing making analysis laborious. We therefore investigated the use of a consumer level scanner to facilitate seed size measurements in conjunction with open source image-processing software.

Results

By using the transmitted light from the slide scanning function of a flatbed scanner and particle analysis of the resulting images, we have developed a method for the rapid and high throughput

SUBMITTER: Herridge RP 

PROVIDER: S-EPMC3046896 | biostudies-literature | 2011 Feb

REPOSITORIES: biostudies-literature