Microarray analysis of differential gene expression profile in peripheral blood cells of patients with human essential hypertension.

ABSTRACT: The polygenic nature of essential hypertension and its dependence on environmental factors pose a challenge for biomedical research. We hypothesized that the analysis of gene expression profiles from peripheral blood cells would distinguish patients with hypertension from normotensives. In order to test this, total RNA from peripheral blood cells was isolated. RNA was reversed-transcribed and labeled and gene expression analyzed using significance Analysis Microarrays (Stanford University, CA, USA). Briefly, Significance Analysis Microarrays (SAM) thresholding identified 31 up-regulated and 18 down-regulated genes with fold changes of ≥2 or ≤0.5 and q-value≤5% in expression. Statistically significantly gene ontology (GO) function and biological process differentially expressed in essential

SUBMITTER: Korkor MT 

PROVIDER: S-EPMC3047082 | biostudies-literature | 2011 Feb

REPOSITORIES: biostudies-literature