Revisiting rat spermatogenesis with MALDI imaging at 20-microm resolution.

ABSTRACT: Matrix-assisted laser desorption/ionization (MALDI) molecular imaging technology attracts increasing attention in the field of biomarker discovery. The unambiguous correlation between histopathology and MALDI images is a key feature for success. MALDI imaging mass spectrometry (MS) at high definition thus calls for technological developments that were established by a number of small steps. These included tissue and matrix preparation steps, dedicated lasers for MALDI imaging, an increase of the robustness against cell debris and matrix sublimation, software for precision matching of molecular and microscopic images, and the analysis of MALDI imaging data using multivariate statistical methods. The goal of these developments is to approach single cell resolution with imaging MS. Currently,

SUBMITTER: Lagarrigue M 

PROVIDER: S-EPMC3047165 | biostudies-literature | 2011 Mar

REPOSITORIES: biostudies-literature