Generation and analysis of expressed sequence tags (ESTs) for marker development in yam (Dioscorea alata L.).

ABSTRACT:

Background

Anthracnose (Colletotrichum gloeosporioides) is a major limiting factor in the production of yam (Dioscorea spp.) worldwide. Availability of high quality sequence information is necessary for designing molecular markers associated with resistance. However, very limited sequence information pertaining to yam is available at public genome databases. Therefore, this collaborative project was developed for genetic improvement and germplasm characterization of yams using molecular markers. The current investigation is focused on studying gene expression, by large scale generation of ESTs, from one susceptible (TDa 95-0310) and two resistant yam genotypes (TDa 87-01091, TDa 95-0328) challenged with the fungus. Total RNA was isolated from young leaves of resistant and susceptib

SUBMITTER: Narina SS 

PROVIDER: S-EPMC3047301 | biostudies-literature | 2011 Feb

REPOSITORIES: biostudies-literature