Soluble guanylate cyclase alpha1beta1 limits stroke size and attenuates neurological injury.

ABSTRACT:

Background and purpose

Nitric oxide mediates endothelium-dependent vasodilation, modulates cerebral blood flow, and determines stroke outcome. Nitric oxide signals in part by stimulating soluble guanylate cyclase (sGC) to synthesize cGMP. To study the role of sGC in stroke injury, we compared the outcome of cerebral ischemia and reperfusion in mice deficient in the alpha(1) subunit of sGC (sGCalpha(1)(-/-)) with that in wild-type mice.

Methods

Blood pressure, cerebrovascular anatomy, and vasoreactivity of pressurized carotid arteries were compared in both mouse genotypes. Cerebral blood flow was measured before and during middle cerebral artery occlusion and reperfusion. We then assessed neurological deficit and infarct volume after 1 hour of occlusion and 23 hours of reperf

SUBMITTER: Atochin DN 

PROVIDER: S-EPMC3047459 | biostudies-literature | 2010 Aug

REPOSITORIES: biostudies-literature