Continuous-time random walk models of DNA electrophoresis in a post array: part II. Mobility and sources of band broadening.

ABSTRACT: Using the two-state, continuous-time random walk model, we develop expressions for the mobility and the plate height during DNA electrophoresis in an ordered post array that delineate the contributions due to (i) the random distance between collisions and (ii) the random duration of a collision. These contributions are expressed in terms of the means and variances of the underlying stochastic processes, which we evaluate from a large ensemble of Brownian dynamics simulations performed using different electric fields and molecular weights in a hexagonal array of 1 μm posts with a 3 μm center-to-center distance. If we fix the molecular weight, we find that the collision frequency governs the mobility. On the contrary, the average collision duration is the most important factor for predicting

SUBMITTER: Olson DW 

PROVIDER: S-EPMC3047477 | biostudies-literature | 2011 Feb

REPOSITORIES: biostudies-literature