Differential Inductive Signaling of CD90 Prostate Cancer-Associated Fibroblasts Compared to Normal Tissue Stromal Mesenchyme Cells.

ABSTRACT:

Unlabelled

Prostate carcinomas are surrounded by a layer of stromal fibroblastic cells that are characterized by increased expression of CD90. These CD90(+) cancer-associated stromal fibroblastic cells differ in gene expression from their normal counterpart, CD49a(+)CD90(lo) stromal smooth muscle cells; and were postulated to represent a less differentiated cell type with altered inductive properties. CD90(+) stromal cells were isolated from tumor tissue specimens and co-cultured with the pluripotent embryonal carcinoma cell line NCCIT in order to elucidate the impact of tumor-associated stroma on stem cells, and the 'cancer stem cell.' Transcriptome analysis identified a notable decreased induction of smooth muscle and prostate stromal genes such as PENK, BMP2 and ChGn compared to

SUBMITTER: Pascal LE 

PROVIDER: S-EPMC3047627 | biostudies-literature | 2011 Jan

REPOSITORIES: biostudies-literature