Microdialysis combined with proteomics for protein identification in breast tumor microenvironment in vivo.

ABSTRACT:

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Tumor microenvironment constitutes a reservoir for proteins released from tumor cells and the host, which can contribute significantly to tumor growth and invasion. This study aims to apply a method of combining in vivo microdialysis and proteomics to identify proteins in mammary tumor interstitial fluids, a major component of tumor microenvironment. In vivo microdialysis was performed in polyomavirus middle T antigen (PyVmT) transgenic mouse mammary tumors and age-matched control wild-type mammary glands. Over four hundred proteins were identified from the microdialysis perfusates, using the Multidimensional Protein Identification Technology. Osteopontin (OPN) is one of the proteins overexpressed in breast tumor perfusates, as confirmed with immunoassays. OPN was also f

SUBMITTER: Xu BJ 

PROVIDER: S-EPMC3047629 | biostudies-literature | 2010 Apr

REPOSITORIES: biostudies-literature