Multispectral labeling of antibodies with polyfluorophores on a DNA backbone and application in cellular imaging.

ABSTRACT: Most current approaches to multiantigen fluorescent imaging require overlaying of multiple images taken with separate filter sets as a result of differing dye excitation requirements. This requirement for false-color composite imaging prevents the user from visualizing multiple species in real time and disallows imaging of rapidly moving specimens. To address this limitation, here we investigate the use of oligodeoxyfluoroside (ODF) fluorophores as labels for antibodies. ODFs are short DNA-like oligomers with fluorophores replacing the DNA bases and can be assembled in many colors with excitation at a single wavelength. A DNA synthesizer was used to construct several short ODFs carrying a terminal alkyne group and having emission maxima of 410-670 nm. We developed a new approach to antibod

SUBMITTER: Guo J 

PROVIDER: S-EPMC3048162 | biostudies-literature | 2011 Mar

REPOSITORIES: biostudies-literature