Metabolic reconstruction of the archaeon methanogen Methanosarcina Acetivorans.

ABSTRACT:

Background

Methanogens are ancient organisms that are key players in the carbon cycle accounting for about one billion tones of biological methane produced annually. Methanosarcina acetivorans, with a genome size of ~5.7 mb, is the largest sequenced archaeon methanogen and unique amongst the methanogens in its biochemical characteristics. By following a systematic workflow we reconstruct a genome-scale metabolic model for M. acetivorans. This process relies on previously developed computational tools developed in our group to correct growth prediction inconsistencies with in vivo data sets and rectify topological inconsistencies in the model.

Results

The generated model iVS941 accounts for 941 genes, 705 reactions and 708 metabolites. The model achieves 93.3% prediction agre

SUBMITTER: Satish Kumar V 

PROVIDER: S-EPMC3048526 | biostudies-literature | 2011 Feb

REPOSITORIES: biostudies-literature