Chromosome conformation capture uncovers potential genome-wide interactions between human conserved non-coding sequences.

ABSTRACT: Comparative analyses of various mammalian genomes have identified numerous conserved non-coding (CNC) DNA elements that display striking conservation among species, suggesting that they have maintained specific functions throughout evolution. CNC function remains poorly understood, although recent studies have identified a role in gene regulation. We hypothesized that the identification of genomic loci that interact physically with CNCs would provide information on their functions. We have used circular chromosome conformation capture (4C) to characterize interactions of 10 CNCs from human chromosome 21 in K562 cells. The data provide evidence that CNCs are capable of interacting with loci that are enriched for CNCs. The number of trans interactions varies among CNCs; some show interaction

SUBMITTER: Robyr D 

PROVIDER: S-EPMC3049788 | biostudies-literature | 2011 Mar

REPOSITORIES: biostudies-literature