Association between composition of the human gastrointestinal microbiome and development of fatty liver with choline deficiency.

ABSTRACT:

Background & aims

Nonalcoholic fatty liver disease affects up to 30% of the US population, but the mechanisms underlying this condition are incompletely understood. We investigated how diet standardization and choline deficiency influence the composition of the microbial community in the human gastrointestinal tract and the development of fatty liver under conditions of choline deficiency.

Methods

We performed a 2-month inpatient study of 15 female subjects who were placed on well-controlled diets in which choline levels were manipulated. We used 454-FLX pyrosequencing of 16S ribosomal RNA bacterial genes to characterize microbiota in stool samples collected over the course of the study.

Results

The compositions of the gastrointestinal microbial communities changed wi

SUBMITTER: Spencer MD 

PROVIDER: S-EPMC3049827 | biostudies-literature | 2011 Mar

REPOSITORIES: biostudies-literature