Selective killing of Burkitt's lymphoma cells by mBAFF-targeted delivery of PinX1.

ABSTRACT: Increased expression of BAFF (B cell-activating factor belonging to the TNF family) and its receptors has been identified in numerous B-cell malignancies. A soluble human BAFF mutant (mBAFF), binding to BAFF receptors but failing to activate B-lymphocyte proliferation, may function as a competitive inhibitor of BAFF and may serve as a novel ligand for targeted therapy of BAFF receptor-positive malignancies. Pin2/TRF1-interacting protein X1 (PinX1), a nucleolar protein, potently inhibits telomerase activity and affects tumorigenicity. In this study, we generated novel recombinant proteins containing mBAFF, a polyarginine tract 9R and PinX1 (or its C/N terminal), to target lymphoma cells. The fusion proteins PinX1/C-G(4)S-9R-G(4)S-mBAFF and PinX1/C-9R-mBAFF specifically bind and internalize

SUBMITTER: Zhang L 

PROVIDER: S-EPMC3049869 | biostudies-literature | 2011 Feb

REPOSITORIES: biostudies-literature