Differential expression of THOC1 and ALY mRNP biogenesis/export factors in human cancers.

ABSTRACT:

Background

One key step in gene expression is the biogenesis of mRNA ribonucleoparticle complexes (mRNPs). Formation of the mRNP requires the participation of a number of conserved factors such as the THO complex. THO interacts physically and functionally with the Sub2/UAP56 RNA-dependent ATPase, and the Yra1/REF1/ALY RNA-binding protein linking transcription, mRNA export and genome integrity. Given the link between genome instability and cancer, we have performed a comparative analysis of the expression patterns of THOC1, a THO complex subunit, and ALY in tumor samples.

Methods

The mRNA levels were measured by quantitative real-time PCR and hybridization of a tumor tissue cDNA array; and the protein levels and distribution by immunostaining of a custom tissue array containi

SUBMITTER: Dominguez-Sanchez MS 

PROVIDER: S-EPMC3050854 | biostudies-literature | 2011 Feb

REPOSITORIES: biostudies-literature