Genomic library screens for genes involved in n-butanol tolerance in Escherichia coli.

ABSTRACT:

Background

n-Butanol is a promising emerging biofuel, and recent metabolic engineering efforts have demonstrated the use of several microbial hosts for its production. However, most organisms have very low tolerance to n-butanol (up to 2% (v/v)), limiting the economic viability of this biofuel. The rational engineering of more robust n-butanol production hosts relies upon understanding the mechanisms involved in tolerance. However, the existing knowledge of genes involved in n-butanol tolerance is limited. The goal of this study is therefore to identify E. coli genes that are involved in n-butanol tolerance.

Methodology/principal findings

Using a genomic library enrichment strategy, we identified approximately 270 genes that were enriched or depleted in n-butanol challenge.

SUBMITTER: Reyes LH 

PROVIDER: S-EPMC3050900 | biostudies-literature | 2011 Mar

REPOSITORIES: biostudies-literature