α-Methylacyl-CoA racemase spliced variants and their expression in normal and malignant prostate tissues.

ABSTRACT:

Objectives

To evaluate the possibility of using α-methylacyl-CoA racemase (AMACR) variants to improve the specificity of prostate cancer (CaP) detection. AMACR has been used as a diagnostic biomarker for CaP and is now a standard biomarker for needle biopsy specimens with ambiguous lesions.

Methods

We used in silico analysis and molecular cloning to discover new AMACR variants and quantitative reverse transcription-polymerase chain reaction (RT-PCR) to measure the transcript levels of AMACR and its variants in 4 prostate cell lines and in 23 pairs of CaP and adjacent normal tissue.

Results

We found 4 novel variants, IAs, IBL, IBLd, and IBLi. Transcript levels of most AMACR variants were significantly upregulated in CaP compared with its adjacent normal counterparts. A

SUBMITTER: Ouyang B 

PROVIDER: S-EPMC3051191 | biostudies-literature | 2011 Jan

REPOSITORIES: biostudies-literature