Biomechanical properties and innervation of the female caveolin-1-deficient detrusor.

ABSTRACT:

Background and purpose

Caveolin-1-deficiency is associated with substantial urogenital alterations. Here, a mechanical, histological and biochemical characterization of female detrusors from wild-type and caveolin-1-deficient (KO) mice was made to increase the understanding of detrusor changes caused by lack of caveolae.

Experimental approach

Length-tension relationships were generated, and we recorded responses to electrical field stimulation, the muscarinic receptor agonist carbachol and the purinoceptor agonist ATP. Tyrosine nitration and the contents of caveolin-1, cavin-1, muscarinic M₃ receptors, phospholipase C(β1), muscle-specific kinase (MuSK) and L-type Ca(2+) channels were determined by immunoblotting. Innervation was assessed by immunohistochemistry.

Key resul

SUBMITTER: Sadegh MK 

PROVIDER: S-EPMC3051387 | biostudies-literature | 2011 Mar

REPOSITORIES: biostudies-literature