Chemical fingerprinting and quantitative analysis of a Panax notoginseng preparation using HPLC-UV and HPLC-MS.

ABSTRACT:

Background

Xuesaitong (XST) injection, consisting of total saponins from Panax notoginseng, was widely used for the treatment of cardio- and cerebro-vascular diseases in China. This study develops a simple and global quality evaluation method for the quality control of XST.

Methods

High performance liquid chromatography-ultraviolet detection (HPLC-UV) was used to identify and quantify the chromatographic fingerprints of the XST injection. Characteristic common peaks were identified using HPLC with photo diode array detection/electrospray ionization tandem mass spectrometry (HPLC-PDA/ESI-MSn).

Results

Representative fingerprints from ten batches of samples showed 27 'common saponins' all of which were identified and quantified using ten reference saponins.

Conclusio

SUBMITTER: Yao H 

PROVIDER: S-EPMC3052241 | biostudies-literature | 2011 Feb

REPOSITORIES: biostudies-literature