Transcriptional profiling of CD133(+) cells in coronary artery disease and effects of exercise on gene expression.

ABSTRACT:

Background aims

Bone marrow (BM)-derived progenitor cells are under investigation for cardiovascular repair but may be altered by disease. Our aim was to identify differences in gene expression in CD133(+) cells of patients with coronary artery disease (CAD) and healthy controls, and determine whether exercise modifies gene expression.

Methods

CD133(+) cells were flow-sorted from 10 CAD patients and four controls, and total RNA was isolated for microarray-based gene expression profiling. Genes that were found to be differentially regulated in patients were analyzed further to investigate whether exercise had any normalizing effect on CD133(+) cells in CAD patients following 3 months of an exercise program.

Results

Improvement in effort tolerance and increases in the n

SUBMITTER: Liu D 

PROVIDER: S-EPMC3052778 | biostudies-literature | 2011 Feb

REPOSITORIES: biostudies-literature