Crystallization and preliminary X-ray analysis of 4-coumarate:CoA ligase from Arabidopsis thaliana.

ABSTRACT: 4-Coumarate:CoA ligase 2 (4CL2) from Arabidopsis thaliana catalyzes the ATP-dependent formation of the 4-coumaroyl-CoA thioester through the formation of 4-coumarate-AMP. Recombinant 4CL2 protein was expressed in Escherichia coli and crystallized by the sitting-drop vapour-diffusion method. The crystals belonged to space group P2(1), with unit-cell parameters a=91.6, b=55.5, c=124.4 Å, α=γ=90.0, β=111.1°.

SUBMITTER: Morita H 

PROVIDER: S-EPMC3053174 | biostudies-literature | 2011 Mar

REPOSITORIES: biostudies-literature