HIV integration targeting: a pathway involving Transportin-3 and the nuclear pore protein RanBP2.

ABSTRACT: Genome-wide siRNA screens have identified host cell factors important for efficient HIV infection, among which are nuclear pore proteins such as RanBP2/Nup358 and the karyopherin Transportin-3/TNPO3. Analysis of the roles of these proteins in the HIV replication cycle suggested that correct trafficking through the pore may facilitate the subsequent integration step. Here we present data for coupling between these steps by demonstrating that depletion of Transportin-3 or RanBP2 altered the terminal step in early HIV replication, the selection of chromosomal sites for integration. We found that depletion of Transportin-3 and RanBP2 altered integration targeting for HIV. These knockdowns reduced HIV integration frequency in gene-dense regions and near gene-associated features, a pattern that

SUBMITTER: Ocwieja KE 

PROVIDER: S-EPMC3053352 | biostudies-literature | 2011 Mar

REPOSITORIES: biostudies-literature